Bio

LARRY D. ACKERMAN

Telephone: (415) 476-4400

Email: larry.ackerman@ucsf.edu

www.ucsf.edu

San Francisco, CA

Technical Skills:

• Tissues Procured : cell, bacteria, viruses, mineralized, vertebrate (including human), invertebrate
• Fixation : perfusion and immersion, freezing, high pressure freezing
• Embedding : Epon, Araldite, Spurrs, Bioplastic, Lowicryl, paraffin and others
• Sectioning : 50 nm --150um including ultrathin, cryo, semi-thin, paraffin, Vibratome
• Stains & Immuno-labeling: general & specific stains, immunofluorescent & EM metals
• Digital imaging : acquisition, processing, animation, scanning, live cell
• Animal Care: mouse colony management
• Computers: General maintenance Windows/DOS, Mac, color management, Web development
• Laboratory Management: purchasing, inventory, equipment operation, maintenance and repairs
• Design: special equipment as needed (eg. TEM low-Z cryo stage, confocal microscope interface)
• Writing: scientific publications
• Supervision and Teaching
• Ability to work and conduct research independently

Microscopes used, maintained and taught :

• Light Microscopes : stereozoom, brightfield, phase-contrast, Nomarski, DIC, polarized, epi-fluorescence, darkfield, CCD cameras (AO, Leitz, Zeiss, Nikon, Olympus, Wild)
• Confocal Microscopes: BioRad MRC 600, 1024 Multi-Photon with Coherent Ti/S Verdi/Mira laser, Leica TCS-SP2, Zeiss Pascal
• Transmission Electron Microscopes: Siemens 1A, 101, JEOL 100b, 100C with SEM/STEM/EDS, Hitachi 7000, Philips 300, 400
• Medium Voltage Transmission Electron Microscopes: Philips 430, FEI T-20
• Scanning Electron Microscopes : Cambridge S4, JEOL JSM-35

Employment History:

Associate Specialist: electron microscopy 2005—present

Departments of Anatomy, Physical Therapy & Rehabilitation, and Pathology at UCSF

Assistant Specialist: light & confocal microscopy 2004—2005

Dr. Hilary Beggs

Department of Ophthalmology

University of California at San Francisco

Specialist: cryo-electron microscopy 2003-2004

Drs. David Agard & John Sedat

Department of Biochemistry & Biophysics

University of California at San Francisco

Senior Research Associate: light, confocal & electron microscopy 1983-2003

Drs. Lily & Yuh Nung Jan

Howard Hughes Medical Institute

Departments of Biochemistry & Biophysics, Physiology & Neuroscience

University of California at San Francisco

Electron Microscopist 1971-1983

Research & Development Service Core Laboratory

Veterans Administration Medical Center, University of Iowa, Iowa City, Iowa

EDUCATION:

Bachelor of Arts in General Science (physics, math, zoology) University of Iowa, 1978

CERTIFICATION:

Certified Electron Microscopist, Microscopy Society of America, Certificate # 0535

PUBLICATIONS:

Hong Y., Ackerman L., Jan L.Y., Jan Y.N. , Distinct roles of Bazooka and Stardust in the specification of Drosophilaphotoreceptor membrane architecture, Proc Natl Acad Sci, 100(22):12712-7. (2003 Oct 28).

Lu, B., Ackerman, L., Jan, L.Y., & Jan, Y.N., Modes of Protein Movement that Lead to the Asymmetric Localization of Partner of Numb during Neuroblast Division in Drosophila, Molecular Cell, 4: 883-891 (1999).

Luo, L., Hensch, T.K., Ackerman, L., Barbel, S., Jan, L.Y., & Jan, Y.N., (1996) Differential effects of the Rac GTPase on Purkinje cell axons and dendritic trunks and spines, Nature 379:837-840. (February 29, 1996)

Jongens, T.A., Ackerman, L.D., Swedlow, J.R., Jan, L.Y., Jan, Y.N., (1994) Germ cell-less encodes a cell-type specific nuclear pore associated protein and functions in the germ cell specification pathway of Drosophila, Genes and Development, 8:2123-2126.

Jarman, A.P., Grell, E.H., Ackerman, L., Jan, L.Y. & Jan, Y.N., (1994) atonal is the proneural gene for Drosophila photoreceptors, Nature, 369: 398-400.

Ackerman, L. D., Jansen, W. T., (1990). A film recording and image processing system for confocal microscopy. Electron Microscopy 1990, Vol. 3: Biological Sciences., Peachey, L. D., Williams, D. B., ed. 798-799. (Proceedings of the XIIth International Congress for Electron Microscopy) San Francisco Press.

Bier, E., Vaessin, H., Shepherd, S., Lee, K., McCall, K., Barbel, S., Ackerman, L., Carretto, R., Uemura, T., Grell, E., Jan, L.Y., and Jan, Y.N. (1989). Searching for Pattern and Mutation in the Drosophila Genome with a P? lacZ Vector, Genes and Development, 3:1273-1287.

Uemura, T., Shepherd, S., Ackerman, L., Jan, L.Y. and Jan, Y.N. (1989). numb, a gene required in determination of cell fate during sensory organ formation in Drosophila embryo, Cell, 58:349-360.

Hay, B., Ackerman, L., Barbel, S., Jan, L. and Jan, Y.N. (1988). Identification of a component of Drosophila polar granules, Development 103: 625-640.

Bier, E., Ackerman, L., Barbel, S., Jan, L. and Jan, Y.N. (1988). Identification and characterization of a neuron-specific nuclear antigen in Drosophila, Science 240: 913-916.

Ackerman,L., & Hunsicker, L. G., (1982) Freeze-Dried Resinless Sections for the Examination of Biological Gels, Electron Microscopy 1982, Vol 3, 535-536, (10th International Congress on Electron Microscopy), Deutsche Gesellschaft fur Elektroenmikroskopie e. V.

Green, A. L. W., Meek, E. S., White, D. W., Stevens, R. H., Ackerman, L. D., Judisch, G. F., & Patil, S. R., (1979) Retinoblastoma Y79 Cell Line: A Study of Membrane Structures, Graefes Archiv fur klinische und experimentelle Opthalmologie, 211:279-287.

Ackerman, L. D., Piros, J., de Carle, D. & Christensen, J., A scanning electron microscopic study of esophageal mucosa, (1976) Proceedings of Workshop on Advances in Biomedical Applications of the SEM, Chicago, IL, Scanning Electron Microscopy/1976, Part V, 247-252.

Ackerman, L.D., Cocks, K.A., & Caughey, R.C., Vibration isolation for ultramicroscopy, (1975), 33rd Annual Proceedings of Electron Microscopy Society of America, Las Vegas, NV, Bailey, G. W. ed., 622-623.

Ackerman, L.D., & Wei, S.H.Y., High Voltage Electron Microscopy of Ion-Milled Dental Enamel, (1974), 32nd Annual Proceedings of Electron Microscopy Society of America, St Louis, Mo, Arceneaux, C.J., ed., 60-61.

Other Media:

Ackerman, L., Lu, B., Jan, L.Y., & Jan, Y.N ., Asymmetric protein localization in Drosophila neural stem cells, a Quicktime movie on the CD “GFP in Motion 2” 2001, Elsevier Science