RESUME
LARRY D. ACKERMAN
PERSONAL DATA
Citzenship Status: USA
Employer: Howard Hughes Medical Institute
University of California at San Francisco (UCSF)
San Francisco, CA 94143
Telephone: (415) 476-8751 FAX: (415) 476-5774
Email: mishot@itsa.ucsf.edu
Website: http://www.ucsf.edu/jan
VOCATIONAL EXPERIENCE:
1971--1983 Electron Microscopist
Research & Development Service
Veterans Administration Medical Center
University of Iowa
Iowa City, Iowa 52240
1983--present Senior Technician/Research Associate
Drs. Lily & Yuh Nung Jan
Howard Hughes Medical Institute
University of California at San Francisco
Departments of Biochemistry & Biophysics, Physiology & Neuroscience
EDUCATION
Bachelor of Arts in General Science (physics, mathematics, zoology)
Degree awarded December, 1978, University of Iowa, Iowa City, Iowa
CERTIFICATION
Certified Electron Microscopist, Electron Microscopy Society of America,
Certificate Number 0535, 1981.
MEMBERSHIPS
Electron Microscopy Society of America, 1973--
Iowa Microbeam Society, 1976--1983
Founding Director, 1976
Secretary, 1977-1978
Program Co-Chairperson, 1976--1978
Microbeam Analysis Society, 1975--1995
Northern California Society for Microscopy, 1983--
PUBLICATIONS
Lu, B., Ackerman, L., Jan, L.Y., & Jan, Y.N., Modes of Protein Movement that Lead to the Asymmetric Localization of Partner of Numb during Neuroblast Division in Drosophila, Molecular Cell, 4: 883-891 (1999).
Luo, L., Hensch, T.K., Ackerman, L., Barbel, S., Jan, L.Y., & Jan, Y.N., (1996) Differential effects of the Rac GTPase on Purkinje cell axons and dendritic trunks and spines, Nature 379:837-840. (February 29, 1996)
Jongens, T.A., Ackerman, L.D., Swedlow, J.R., Jan, L.Y., Jan, Y.N., (1994) Germ cell-less encodes a cell-type specific nuclear pore associated protein and functions in the germ cell specification pathway of Drosophila, Genes and Development, 8:2123-2126.
Jarman, A.P., Grell, E.H., Ackerman, L., Jan, L.Y. & Jan, Y.N., (1994) atonal is the proneural gene for Drosophila photoreceptors, Nature, 369: 398-400.
Ackerman, L. D., Jansen, W. T., (1990). A film recording and image processing system for confocal microscopy. Electron Microscopy 1990, Vol. 3: Biological Sciences., Peachey, L. D., Williams, D. B., ed. 798-799. (Proceedings of the XIIth International Congress for Electron Microscopy) San Francisco Press.
Bier, E., Vaessin, H., Shepherd, S., Lee, K., McCall, K., Barbel, S., Ackerman, L., Carretto, R., Uemura, T., Grell, E., Jan, L.Y., and Jan, Y.N. (1989). Searching for Pattern and Mutation in the Drosophila Genome with a P lacZ Vector, Genes and Development, 3:1273-1287.
Uemura, T., Shepherd, S., Ackerman, L., Jan, L.Y. and Jan, Y.N. (1989). numb, a gene required in determination of cell fate during sensory organ formation in Drosophila embryo, Cell, 58:349-360.
Hay, B., Ackerman, L., Barbel, S., Jan, L. and Jan, Y.N. (1988). Identification of a component of Drosophila polar granules, Development 103: 625-640.
Bier, E., Ackerman, L., Barbel, S., Jan, L. and Jan, Y.N. (1988). Identification and characterization of a neuron-specific nuclear antigen in Drosophila, Science 240: 913-916.
Ackerman,L., & Hunsicker, L. G., (1982) Freeze-Dried Resinless Sections for the Examination of Biological Gels, Electron Microscopy 1982, Vol 3, 535-536, (10th International Congress on Electron Microscopy), Deutsche Gesellschaft fur Elektroenmikroskopie e. V.
Green, A. L. W., Meek, E. S., White, D. W., Stevens, R. H., Ackerman, L. D., Judisch, G. F., & Patil, S. R., (1979) Retinoblastoma Y79 Cell Line: A Study of Membrane Structures, Graefes Archiv fur klinische und experimentelle Opthalmologie, 211:279-287.
Ackerman, L. D., Piros, J., de Carle, D. & Christensen, J., A scanning electron microscopic study of esophageal mucosa, (1976) Proceedings of Workshop on Advances in Biomedical Applications of the SEM, Chicago, IL, Scanning Electron Microscopy/1976, Part V, 247-252.
Ackerman, L.D., Cocks, K.A., & Caughey, R.C., Vibration isolation for ultramicroscopy, (1975), 33rd Annual Proceedings of Electron Microscopy Society of America, Las Vegas, NV, Bailey, G. W. ed., 622-623.
Ackerman, L.D., & Wei, S.H.Y., High Voltage Electron Microscopy of Ion-Milled Dental Enamel, (1974), 32nd Annual Proceedings of Electron Microscopy Society of America, St Louis, Mo, Arceneaux, C.J., ed., 60-61.
Micrographs published in books:
Biomedical Research Applications of Scanning Electron Microscopy, Vol. 1, Hodges, G., Hallowes, R., ed., (1979) Academic Press, London.
Electron Microscopy in Human Medicine, Vol 7, Johannessen, J. V. ed., (1980), p. 95, McGraw-Hill.
Scientific Foundations of Gastroenterology, Sircus, W., Smith, A., ed., (1980), p. 1,
W. B. Saunders, Philadelphia.
Educational Assignments:
Microscopy Societry of America (formerly: Electron Microscopy Society of America)
Annual Meetings 1973, 1974, 1975, 1979, 1989, 1991, 1994, 1999
International Congress on Electron Microscopy
1978--Toronto, 1982--Hamburg, 1990--Seattle
Scanning Electron Microscopy Symposiums and Workshops
1973, 1974, 1976, 1977
Electron Microscopy Colloquium, Iowa State University
1973--1979, 1981--1983
Iowa Microbeam Society Annual Meetings
1976--1982
Methods and Techniques:
Tissues Procured: cell, bacterial & viral cultures, mineralized, vertebate (including human), invertebrate, surgical and microscopic dissection
Fixation: perfusion and immersion with various solutions, freezing, high pressure freezing
Dehydration: ethanol and various other agents
Embedding: Epon, Araldite, Spurrs, Bioplastic, Lowicryl, paraffin and others
Sectioning: ultrathin--50-150nm including cryo, semi-thin--.5--3mm, thin--5-12mm paraffin, 8-20mm cryo, 50-200mm Vibratome
Support films: Nitrocellulose, Formvar, carbon, ultra-thin carbon
Stains: toluidine blue, periodic acid-Schiffs, uranyl salts, lead salts, bismuth subnitrate, phosphotungstic acid, Giemsa and other specialized stains
Immuno-labeling: light level labels including horseradish peroxidase, alkaline phosphatase, avidin biotin amplification, fluorescein, Texas Red, Rhodamine, DAPI, Cy-3, CY-5, silver intensification, EM level labels including colloidal gold, 1.4nm gold, horseradish peroxidase, gold-silver enhancement
Photography: copy, macro, micro, 35mm--5" X 7" film, black & white and color, numerous developers including color and special formulations, reduction, intensification, staining, diazos, Cibachromes, reproduction prints, print spotting, labeling, dry mounting, slide duping, slide composites
Digital imaging: acquisition, processing, retouching, compositing, scanning, film recording, graphics creation with bit-mapped and vector based software (Mac, Windows & SGI platforms)
Video: intensifiers, time lapse
Negative staining
Shadow casting
Replication
Cryofracture
Freeze-fracture
Freeze-drying
Ion milling
Critical Point Drying
Autoradiography
Energy and wavelength dispersive X-ray analysis
TEM: brightfield, darkfield, diffraction, goniometer, cold stage
SEM: secondary, backscattered
Computers: Fortran, Pascal, general maintenance Windows/DOS, Mac, color management
Ethernet issues, Web pages, animation, video output
Immuno labeling for light microscopy & electron microscopy
General laboratory equipment: operation, maintenance and small repairs
Design: special equipment as needed (eg. TEM low-Z cryo stage with temperature probe, darkfield stage for macrophotography, new interface for Nikon TE-300 to BioRad 1024 Multi-Photon system
Instruments and equipment used:
Light Microscopes: AO, Leitz, Zeiss, Nikon, Olympus, Wild
stereozoom, brightfield, phase-contrast, Nomarski DIC, polarized,
epi-fluorescence, darkfield, photo systems, video/CCD systems
Confocal Microscopes: BioRad MRC 600, 1024 Multi-Photon with Coherent Ti/S Verdi/Mira laser, Leica TCS-SP2
Transmission Electron Microscopes: Siemens 1A, 101, JEOL 100b, 100C with SEM/STEM/EDS, Hitachi 7000, Philips 300, 400, Tecnai 20
Medium Voltage Transmission Electron Microscopes: Philips 430
High Voltage Transmission Electron Microscopes: AEI-EM 7
Scanning Electron Microscopes: Cambridge S4-10, JEOL JSM-35C, JSM 840A, ISI DS-130
Ultramicrotomes: Reichert Om-U2, Ultracut E, Sorvall MT-2B, MT 5000,
LKB I, IV, V
Cryoultramicrotomes: Reichert FC-4
Cryostats: AO, Slee, Reichert-Jung 2800E, Zeiss Micron
Vibratome 100
Microtomes: Leitz 1512
Knifemaker: LKB
Vacuum evaporators: Denton, Balzers
Sputter Coaters: Technics, Anatech
Critical Point Dryers: Polaron, Denton, Tousimis
Freeze-dryers: Coulter-Terracio
Mineralized tissue diamond saws and grinding wheels: Gillings-Hamco, Bronwill, Buehler
Image Analysers: Quantimet, Zeiss
Computers: IBM PC and clones (DOS & WINDOWS , and OS2), MacIntosh, and some use of Silicon Graphics and UNIX (Sun) machines
Software: VoxelBlast 3-D, Image Pro Plus, Image Tools, CorelDraw, PhotoPaint, Adobe Photshop, Illustrator, word processors, utilities, color managment, network systems, graphics utilities including scanning, printing & film recording, Excel, Access, FileMaker